71, No. 4, pp. 1559-1563, April 1974. A Functional Mitotic Spindle Prepared from Mammalian Cells in Culture. (microtubule assembly/motifity/anaphase in vitro).

The mitotic spindle is a complex and fascinating macromolecular machine that performs a crucial task: distributing an exact complement of chromosomes to each daughter cell during mitosis or meiosis. It is composed primarily of microtubules, dynamic cytoskeletal polymers of α/β tubulin subunits with an intrinsic structural polarity.

Browse. Create. Log in Sign up. Dynein in spindle function. Binds the astoral microtubules to the cell membrane, helping pull the centrosome toward the membrane.

Mitotic spindle function

(2010). Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are required components of mitotic spindles has long been controversial. Gulluni et al. reveal a kinase-independent scaffolding function of PI3K-C2α that affects mitotic spindle formation. Reduced levels of PI3K-C2α reduce tumor growth initially but provide a growth advantage later in mouse models of breast cancer. Loss of PI3K-C2α also increases sensitivity of tumors to taxanes.

The antitumor activity of paclitaxel is based on tubulin-binding and stabilization of non-functional microtubule bundles, thereby blocking normal mitotic spindle

Thus, the checkpoint helps to prevent mitotic errors and to maintain genome integrity. The Saccharomyces cerevisiae mitotic spindle in budding yeast is exemplified by its simplicity and elegance.

Huang, J., Raff, J. W. The disappearance of cyclin B at the end of mitosis is to the spindle poles and has a role in mitotic spindle checkpoint inactivation. J. Cell

Called also mitotic spindle . 3. muscle spindle . mitotic Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field. Keywords cell division light microscopy electron microscopy microtubule function microtubule polymerization dynamics core cell cycle control mechanisms spindle functions relevant to cancer cell-free Xenopus egg extracts system The Prp19 Complex Directly Functions in Mitotic Spindle Assembly Jennifer C. Hofmann1¤, Justus Tegha-Dunghu 1, Stefanie Dräger, Cindy L.Will2, Reinhard Lührmann2, Oliver J. Gruss1* 1 Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany, 2 Department of Cellular Biochemistry, Max A novel tropomyosin isoform functions at the mitotic spindle and Golgi in Drosophila Lauren M. Goins and R. Dyche Mullins Department of Cellular and Molecular Pharmacology, School of Medicine, University of California, San Francisco, San Francisco, CA 94158 ABSTRACT Most eukaryotic cells express multiple isoforms of the actin-binding protein The mitotic spindle is the macromolecular machine that segregates chromosomes to two daughter cells during mitosis.

Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are required components of mitotic spindles has long been controversial.
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However The mitotic spindle is composed of microtubule proteins that slowly increase in length during the The checkpoint kinase 1 (Chk1) is known for its role in DNA damage response, especially the G2/M and the mitotic spindle checkpoint, supporting the idea of an The spindle apparatus is a network of microtubules that forms within a dividing eukaryotic cell.

Using time-course holographic imaging studies, this cell cycle arrest followed by this complex accumulates at the centrosome to support mitotic spindle formation.
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Mitotic spindle: focus on the function of huntingtin. Godin JD(1), Humbert S. Author information: (1)Institut Curie, Orsay, France. Mitotic spindle assembly and orientation are tightly regulated to allow the appropriate segregation of genetic material and cell fate determinants during symmetric and asymmetric divisions.

function are limited to spindles in mitosis and to spindle orientation an d nuclear positioning in the cytoplasm. Microtubule function is regulated in large part via products of the 6 kinesin gene family and the 1 cytoplasmi c dynein gene. A single bipolar kinesin (Cin8, class Kin-5), together The mitotic spindle is the macromolecular machine that segregates chromosomes to two daughter cells during mitosis.

Mitotic progression is orchestrated by the microtubule-based motor dynein, which sustains all mitotic spindle functions. During cell division, cytoplasmic dynein acts with the high-molecular-weight complex dynactin and nuclear mitotic apparatus (NuMA) to organize and position the spindle.

It is composed primarily of microtubules, dynamic cytoskeletal polymers of α/β tubulin subunits with an intrinsic structural polarity.

Spindle checkpoint function is essential for DNA damage-induced mitotic catastrophe Finally, we examined whether spindle checkpoint function is required for the induction of mitotic catastrophe in Microtubule function is regulated in large part via products of the 6 kinesin gene family and the 1 cytoplasmic dynein gene. A single bipolar kinesin (Cin8, class Kin-5), together with a depolymerase (Kip3, class Kin-8) or minus-end-directed kinesin (Kar3, class Kin-14), can support spindle function and cell viability. Live-imaging of mitotic cells expressing EB1-GFP demonstrates that APC(1-1450) compromises the dynamics of EB1-comets, increasing the frequency of EB1-GFP pausing. Together these data provide novel insight into how APC may regulate mitotic spindle function and how errors in chromosome segregation are tolerated in tumor cells. Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division.